Autor(es): Greiser-Wilke I, Moenning V, Kaaden O R, Figueiredo L T

Resumo: Fourteen hybridoma cell lines secreting antibodies against the Semliki Forest virus nucleocapsid protein were established - employed for identification of conserved epitopes among 27 alphavirus types - subtypes. Using an antibody capture test, the antibodies were found to cross-react to variable degree with alphaviruses belonging to the Semliki Forest, western encephalitis - eastern encephalitis complexes, as well as Middelburg - Ndumu. None of the antibodies reacted with either Venezuelan equine encephalitis or Barmah Forest virus. Due to their reactivity with Fort Morgan, Y62-33, Whataroa - chikungunya, the monoclonal antibodies were divided into six reactive types. Competition assays showed that the epitopes for all the types were either identical or clustered on a single domain of the nucleocapsid protein.

Palavras-Chave: Alphaviruses; Nucleocapsid Protein; Mabs

Imprenta: The Journal of General Virology, v. 70, Pt 3, p. 743-748, 1989

Identificador do Objeto Digital: 10.1099/0022-1317-70-3-743

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de Publicação: 1989