Autor(es): Goh Lucas Y H, Hobson-Peters Jody, Prow Natalie A, Gardner Joy, Bielefeldt-Ohmann Helle, Suhrbier Andreas, Hall Roy A

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for epidemics of debilitating arthritic disease. The recent outbreak (2004-2014) resulted in an estimated 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. The development of CHIKV-specific diagnostics - research tools is thus highly desirable. Herein we describe the generation - characterization of the first mAbs specific for the capsid protein (CP) of CHIKV. The antibodies recognized isolates representing the major genotypes of CHIKV, as well as several other alphaviruses, - were reactive in a range of assays including ELISA, Western blot, immunofluorescence - immunohistochemistry (IHC). We have also used the anti-CP mAb 5.5G9 in IHC studies to show that capsid antigen is persistently expressed 30 days post-infection in cells with macrophage morphology in a mouse model of chronic CHIKV disease. These antibodies may thus represent useful tools for further research, including investigations into the structure - function of CHIKV CP, - as valuable reagents for CHIKV detection in a range of settings.

Imprenta: The Journal of General Virology, v. 96, Pt 3, p. 507-512, 2015

Identificador do objeto digital: 10.1099/jgv.0.000002

Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology

Data de publicação: 2015

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