Autor(es): Das Biswadeep, Sahu Abhipsa, Das Mumani, Patra Aparna, Dwibedi Bhagirathi, Kar Santanu K, Hazra Rupenangshu K

Resumo: Chikungunya virus (CHIKV), an arthritogenic alphavirus, is transmitted to humans by mosquitoes of genus Aedes, mainly Aedes aegypti - Aedes albopictus. The resurgence of CHIKV in different parts of India is a point of major public health concern. In 2010, chikungunya outbreaks with high epidemic magnitude were recorded in coastal areas of Orissa, Eastern India, affecting more than 15,000 people coupled with severe arthralgia - prolonged morbidites. Detailed entomological, serological - molecular investigation of this unprecendented outbreak was carried out by collecting - studying 1359 mosquito samples belonging to A. albopictus, A. aegypti, A. vittatus, A. edwardsii - Culex species - 220 patients serum from the affected areas. In this study, CHIKV specific IgM capture-ELISA - reverse-transcription PCR (RT-PCR) were done to detect recent infection of CHIKV in serum samples - adult mosquitoes collected from the affected areas. The high maximum likelihood estimate (MLE) (15.2) in A. albopictus mosquitoes indicated that it was the principal vector involved in transmission of CHIKV in Orissa. Phylogenetic analysis revealed that the CHIKV strains involved in the outbreak belonged to the Indian Ocean Lineage (IOL) group within the East, Central - South African (ECSA) genotype. Genetic characterization of envelope glycoprotein (E1 - E2) genes revealed that all the CHIKV isolates from Orissa had the E1-A226V mutation that enhances viral dissemination - transmissibility by A. albopictus mosquitoes along with E2-L210Q - E2-I211T mutations, which play an epistatic role with E1-A226V mutation in adaptation of CHIKV to A. albopictus by increasing its midgut infectivity, thereby favoring its vectorial capacity. Our results showed the involvement of A. albopictus vector in the recent outbreaks in Orissa - circulation of IOL strains of ECSA genotype of CHIKV with E1-A226V, E2-L210Q - E2-I211T mutations in vectors - patients serum.

Palavras-Chave: Chikungunya; OrissaIndia; Aedes albopictus; RT-PCR; Phylogenetic analysis

Imprenta: Infection, Genetics and Evolution, v. 12, n. 5, p. 1094-1101, 2012

Identificador do Objeto Digital: 10.1016/j.meegid.2012.03.012

Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de Publicação: 2012