Molecular genome tracking of East, Central and South African genotype of Chikungunya virus in South-east Asia between 2006 - 2009
Autor(es): Suwannakarn Kamol, Theamboonlers Apiradee, Poovorawan Yong
Resumo: To underst- the epidemiology of the East, Central - South African (ECSA) genotype of Chikungunya virus (CHIKV) in terms of emerging - re-emerging infections, this study has been aimed at investigating the evolutionary parameters, genomic signatures - molecular tracking of the CHIKV ECSA genotype in South-east Asia - coastal areas of the Indian Ocean between 2006 - 2009 by using phylogenetic analysis - the Bayesian Markov Chain Monte Carlo (BMCMC) evolutionary estimation. Nearly complete genome sequences of 53 CHIKV isolates from all genotypes were subjected to phylogenetic analysis - evolutionary parameter estimation. The amino acids of 67 of ECSA genotype during 2006 to 2009 were compared for finding molecular signature tracking. The ECSA genotype signatures were visualized to find the possible transmission root was projected onto a geographic map. Phylogenetic analysis showed the ECSA genotype was divided into 2 groups. The first group comprises viruses from India - Southeast Asian countries. The second group consists of strains typically circulating in Sri Lanka in 2008. The evolutionary parameters of these groups depicted the time of the most recent common ancestor at approximately 7.5 years ago. The genomic signatures revealed the positions of amino acid variation in each group. The molecular evolution projected onto a geographical map showed the routes of CHIKV transmission from 2006 to 2009. Molecular tracking will assist in underst-ing transmission routes, epidemiology - molecular evolution of CHIKV.
Palavras-Chave: Chikungunya virus; East Central and South African genotype; Genomic signature; Evolutionary parameter
Imprenta: Asian Pacific Journal of Tropical Medicine, v. 4, n. 7, p. 535-540, 2011
Identificador do objeto digital: 10.1016/S1995-7645(11)60141-7
Descritores: Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology
Data de publicação: 2011
