Molecular characterization of Chikungunya virus isolates from clinical samples and adult Aedes albopictus mosquitoes emerged from larvae from Kerala, South India
Autor(es): Niyas Kudukkil P, Abraham Rachy, Unnikrishnan Ramakrishnan Nair, Mathew Thomas, Nair Sajith, Manakkadan Anoop, Issac Aneesh, Sreekumar Easwaran
Resumo: Chikungunya virus (CHIKV), an arthritogenic alphavirus, is transmitted to humans by infected Aedes (Ae.) aegypti - Ae.albopictus mosquitoes. In the study, reverse-transcription PCR (RT PCR) - virus isolation detected CHIKV in patient samples - also in adult Ae.albopictus mosquitoes that was derived from larvae collected during a chikungunya (CHIK) outbreak in Kerala in 2009. The CHIKV strains involved in the outbreak were the East, Central - South African (ECSA) genotype that had the E1 A226V mutation. The viral strains from the mosquitoes - CHIK patients from the same area showed a close relationship based on phylogenetic analysis. Genetic characterization by partial sequencing of non-structural protein 2 (nsP2; 378 bp), envelope E1 (505 bp) - E2 (428 bp) identified one critical mutation in the E2 protein coding region of these CHIKV strains. This novel, non-conservative mutation, L210Q, consistently present in both human - mosquito-derived samples studied, was within the region of the E2 protein (amino acids E2 200-220) that determines mosquito cell infectivity in many alpha viruses. Our results show the involvement of Ae. albopictus in this outbreak in Kerala - appearance of CHIKV with novel genetic changes. Detection of virus in adult mosquitoes, emerged in the laboratory from larvae, also points to the possibility of transovarial transmission (TOT) of mutant CHIKV strains in mosquitoes.
Imprenta: Virology Journal, v. 7, p. 189, 2010
Identificador do objeto digital: 10.1186/1743-422X-7-189
Descritores: Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health
Data de publicação: 2010
