Autor(es): M Naresh Kumar C V, Anthony Johnson A M, R Sai Gopal D V

Resumo: Chikungunya virus has caused numerous large outbreaks in India. Suspected blood samples from the epidemic were collected - characterized for the identification of the responsible causative from Rayalaseema region of -hra Pradesh. RT-PCR was used for screening of suspected blood samples. Primers were designed to amplify partial E1 gene - the amplified fragment was cloned - sequenced. The sequence was analyzed - compared with other geographical isolates to find the phylogenetic relationship. The sequence was submitted to the Gen bank DNA database (accession DQ888620). Comparative nucleotide homology analysis of the AP Ra-CTR isolate with the other isolates revealed 94.7+/-3.6 per cent of homology of CHIKAPRa-CTR with other isolates of Chikungunya virus at nucleotide level - 96.8+/-3.2 per cent of homology at amino acid level. The current epidemic was caused by the Central African genotype of CHIKV, grouped in Central Africa cluster in phylogenetic trees generated based on nucleotide - amino acid sequences.

Imprenta: The Indian Journal of Medical Research, v. 126, n. 6, p. 534-540, 2007

Descritores: Chikungunya virus - DNA ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2007

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