Autor(es): Soni Manisha, Singh Anil Kumar, Sharma Shashi, Agarwal Ankita, Gopalan Natarajan, Rao P V Lakshmana, Parida Manmohan, Dash Paban Kumar

Resumo: Chikungunya (CHIK) fever is one of the most important arboviral infections of medical significance. The objective of the present study is to identify - characterize the etiology of a focal febrile arthritis outbreak from Gwalior, northern India, during October-November 2010. A detailed virological (isolation) - molecular (end-point RT-PCR, quantitative RT-PCR, - nucleotide sequencing) investigation of this outbreak was carried out by collecting - studying 52 clinical samples - 15 mosquito pools from the affected region. The investigation revealed the presence of CHIK viral RNA in 29% of clinical samples - 13% mosquito pool by RT-PCR. The quantification of CHIK viral RNA in samples varied from 10(2.50) to 10(6.67) copies/mL, as demonstrated through quantitative RT-PCR. In addition, six CHIK viruses were isolated from RT-PCR positive samples. The nucleotide sequences of partial E1 gene of five representative CHIK viruses were deciphered, which revealed that all the viral strains from this outbreak belong to the recently emerging ECS African genotype. Identification of Chikungunya virus ECSA African genotype as the etiology of the present outbreak confirms the continued circulation of the novel genotype, since 2006, in India. The identification of CHIK virus in Aedes aegypti also confirmed it as the major vector in northern India.

Imprenta: The Scientific World Journal, v. 2013, p. 367382, 2013

Identificador do objeto digital: 10.1155/2013/367382

Descritores: Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2013

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