Mechanism of increased dissemination of Chikungunya virus in Aedes albopictus mosquitoes concurrently ingesting microfilariae of Dirofilaria immitis
Autor(es): Zytoon E M, el-Belbasi H I, Matsumura T
Resumo: We investigated whether concurrent ingestion of chikungunya virus - microfilariae of Dirofilaria immitis increases viral dissemination - multiplication in a mosquito vector. The increased rate of dissemination of this virus in mosquitoes concurrently ingesting both agents was found when homogenates of bodies - those of legs only were examined. It was significantly higher than that of controls ingesting the virus alone through the end of the experiment on day 14 after infection. We next studied the mechanism by which the presence of microfilariae enabled the virus to enter into the hemocoel - to reach the salivary gl-s. We checked our results using histopathologic procedures - electron microscopy by identifying holes produced by the microfilariae that penetrated the midgut epithelial layer. When the midgut of mosquitoes was punctured with a thin needle immediately after the mosquitoes ingested viruses, higher infection rates were observed than in mosquitoes without such punctures.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 49, n. 2, p. 201-207, 1993
Identificador do objeto digital: 10.4269/ajtmh.1993.49.201
Descritores: Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 1993
