Autor(es): Sreejith R, Rana Jyoti, Dudha Namrata, Kumar Kapila, Gabrani Reema, Sharma Sanjeev K, Gupta Amita, Vrati Sudhanshu, Chaudhary Vijay K, Gupta Sanjay

Resumo: The four nonstructural proteins (nsPs1-4) of Chikungunya virus (CHIKV) play important roles involving enzymatic activities - specific interactions with both viral - host components, during different stages of viral pathogenesis. Elucidation of the presence -/or absence of interactions among nsPs in a systematic manner is thus of scientific interest. In the current study, each pair-wise combination among the four nonstructural proteins of CHIKV was systematically analyzed for possible interactions. Six novel protein interactions were identified for CHIKV, using systems such as yeast two-hybrid, GST pull down - ELISA, three of which have not been previously reported for the genus Alphavirus. These interactions form a network of organized associations that suggest the spatial arrangement of nonstructural proteins in the late replicase complex. The study identified novel interactions as well as concurred with previously described associations in related alphaviruses.

Palavras-Chave: Alphaviruses; Chikungunya virus; Nonstructural proteins; Protein interactions; Yeast two-hybrid

Imprenta: Virus Research, v. 169, n. 1, p. 231-236, 2012

Identificador do objeto digital: 10.1016/j.virusres.2012.08.006

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus

Data de publicação: 2012

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