Longitudinal analysis of the human antibody response to Chikungunya virus infection: Implications for serodiagnosis and vaccine development
Autor(es): Kam Yiu-Wing, Lee Wendy W L, Simarmata Diane, Harjanto Sumitro, Teng Terk-Shin, Tolou Hugues, Chow Angela, Lin Raymond T P, Leo Yee-Sin, Rénia Laurent, Ng Lisa F P
Resumo: Chikungunya virus (CHIKV) is an alphavirus which causes chronic - incapacitating arthralgia in humans. Although previous studies have shown that antibodies against the virus are produced during - after infection, the fine specificity of the antibody response against CHIKV is not known. Here, using plasma from patients at different times postinfection, we characterized the antibody response against various proteins of the virus. We have shown that the E2 - E3 glycoproteins - the capsid - nsP3 proteins are targets of the anti-CHIKV antibody response. Moreover, we have identified the different regions in these proteins which contain the linear epitopes recognized by the anti-CHIKV antibodies - determined their structural localization. Data also illustrated the effect of a single K(252)Q amino acid change at the E2 glycoprotein that was able to influence antibody binding - interaction between the antibodies - epitope because of the changes of epitope-antibody binding capacity. This study provides important knowledge that will not only aid in the underst-ing of the immune response to CHIKV infection but also provide new knowledge in the design of modern vaccine development. Furthermore, these pathogen-specific epitopes could be used for future seroepidemiological studies that will unravel the molecular mechanisms of human immunity - protection from CHIKV disease.
Imprenta: Journal of Virology, v. 86, n. 23, p. 13005-13015, 2012
Identificador do objeto digital: 10.1128/JVI.01780-12
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Immune response ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2012
