Longitudinal analysis of natural killer cells in Dengue virus-infected patients in comparison to Chikungunya and Chikungunya/Dengue virus-infected patients
Autor(es): Petitdemange Caroline, Wauquier Nadia, Devilliers Hervé, Yssel Hans, Mombo Illich, Caron Mélanie, Nkoghé Dieudonné, Debré Patrice, Leroy Eric, Vieillard Vincent
Resumo: Dengue virus (DENV) is the most prominent arbovirus worldwide, causing major epidemics in South-East Asia, South America - Africa. In 2010, a major DENV-2 outbreak occurred in Gabon with cases of patients co-infected with chikungunya virus (CHIKV). Although the innate immune response is thought to be of primordial importance in the development - outcome of arbovirus-associated pathologies, our knowledge of the role of natural killer (NK) cells during DENV-2 infection is in its infancy. We performed the first extensive comparative longitudinal characterization of NK cells in patients infected by DENV-2, CHIKV or both viruses. Hierarchical clustering - principal component analyses were performed to discriminate between CHIKV - DENV-2 infected patients. We observed that both activation - differentiation of NK cells are induced during the acute phase of infection by DENV-2 - CHIKV. Combinatorial analysis however, revealed that both arboviruses induced two different signatures of NK-cell responses, with CHIKV more associated with terminal differentiation, - DENV-2 with inhibitory KIRs. We show also that intracellular production of interferon-? (IFN-?) by NK cells is strongly stimulated in acute DENV-2 infection, compared to CHIKV. Although specific differences were observed between CHIKV - DENV-2 infections, the significant remodeling of NK cell populations observed here suggests their potential roles in the control of both infections.
Imprenta: PLoS Neglected Tropical Diseases, v. 10, n. 3, p. e0004499, 2016
Identificador do objeto digital: 10.1371/journal.pntd.0004499
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic
Data de publicação: 2016
