Autor(es): Muruganandam N, Chaaithanya I K, Senthil G S, Shriram A N, Bhattacharya D, Jeevabharathi G S, Sudeep A B, Pradeepkumar N, Vijayachari P

Resumo: Chikungunya virus (CHIKV) is an Alphavirus belonging to the family Togaviridae. In 2006, CHIKV infection struck the -aman - Nicobar archipelago, with an attack rate of 60%. There were more than 10 cases with acute flaccid paralysis simulating the Guillian Barre Syndrome. The majority of the patients presented severe joint pain. The cause for such an explosive nature of the outbreak with increased morbidity was not known. The isolation of CHIKV was attempted - succeeded from nine subjects presenting clinical symptoms of Chikungunya fever. The cDNA of all the isolates was sequenced for partial E1 - nsP1 genes. Sequences were aligned based on the double locus sequence typing concept. The phylogenetic analysis shows that sequences of -aman isolates grouped with the East, Central, - South African genotype of virus isolates from India, Sri Lanka, - Réunion. The genetic distance between -aman isolates - the Réunion isolates was very small. The phylogenetic analysis confirmed the origin of the isolates responsible for the first ever confirmed CHIKV outbreak in these isl-s to be the East, Central, - South African genotype. In this manuscript, we discuss the involvement of the East, Central, - South African strain with the Chikungunya fever outbreak in this archipelago - double locus sequence typing as a first time approach.

Palavras-Chave: Chikungunya virus; Outbreak; Genotyping; DLST; Andaman and Nicobar islands; India

Imprenta: Canadian Journal of Microbiology, v. 57, n. 12, p. 1073-1077, 2011

Identificador do objeto digital: 10.1139/w11-103

Descritores: Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de publicação: 2011

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