"ISA-Lation" of single-stranded positive-sense RNA viruses from non-infectious clinical/animal samples
Autor(es): Aubry Fabien, Nougairède Antoine, de Fabritus Lauriane, Piorkowski Géraldine, Gould Ernest A, de Lamballerie Xavier
Resumo: Isolation of viral pathogens from clinical -/or animal samples has traditionally relied on either cell cultures or laboratory animal model systems. However, virus viability is notoriously susceptible to adverse conditions that may include inappropriate procedures for sample collection, storage temperature, support media - transportation. Using our recently described ISA method, we have developed a novel procedure to isolate infectious single-str-ed positive-sense RNA viruses from clinical or animal samples. This approach, that we have now called ISA-lation", exploits the capacity of viral cDNA subgenomic fragments to re-assemble - produce infectious viral RNA in susceptible cells. Here, it was successfully used to rescue enterovirus, Chikungunya - Tick-borne encephalitis viruses from a variety of inactivated animal - human samples. ISA-lation represents an effective option to rescue infectious virus from clinical -/or animal samples that may have deteriorated during the collection - storage period, but also potentially overcomes logistic - administrative difficulties generated when complying with current health - safety - biosecurity guidelines associated with shipment of infectious viral material."
Imprenta: PloS One, v. 10, n. 9, p. e0138703, 2015
Identificador do objeto digital: 10.1371/journal.pone.0138703
Descritores: Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2015
