Autor(es): Fongsaran Chanida, Jirakanwisal Krit, Kuadkitkan Atichat, Wikan Nitwara, Wintachai Phitchayapak, Thepparit Chutima, Ubol Sukathida, Phaonakrop Narumon, Roytrakul Sittiruk, Smith Duncan R

Resumo: Chikungunya virus (CHIKV), the virus responsible for the disease chikungunya fever in humans, is transmitted by Aedes mosquitoes. While significant progress has been made in underst-ing the process by which CHIKV enters into mammalian cells, far less progress has been made in underst-ing the CHIKV entry process in insect cells. This study sought to identify mosquito-cell-expressed CHIKV-binding proteins through a combination of virus overlay protein binding assays (VOPBA) - mass spectroscopy. A 50-kDa CHIKV-binding protein was identified as the ATP synthase ? subunit (ATPS?). Co-immunoprecipitation studies confirmed the interaction, - colocalization analysis showed cell-surface - intracellular co-localization between CHIKV - ATPS?. Both antibody inhibition - siRNA-mediated downregulation experiments targeted to ATPS? showed a significant reduction in viral entry - virus production. These results suggest that ATPS? is a CHIKV-binding protein capable of mediating the entry of CHIKV into insect cells.

Imprenta: Archives of Virology, v. 159, n. 12, p. 3353-3364

Identificador do objeto digital: 10.1007/s00705-014-2210-4

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de publicação: 2014

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