Autor(es): Sudeep A B, Hundekar S L, Jacob P G, Balasubramanian R, Arankalle V A, Mishra A C

Resumo: To identify the aetiological agent/s of an outbreak of chikungunya-like illness with high morbidity - several fatalities in Tamil Nadu, India, 2009-2010. Two hundred - seventeen serum samples were collected from the affected areas - screened for chikungunya virus (CHIKV), dengue virus (DENV) - Japanese encephalitis virus (JEV) IgM antibodies using MAC-ELISA kits. A few selected samples were also tested for Ross River, Sindbis, - Murrey Valley viruses by RT-PCR - Hantan virus by serology. Twelve acute serum - mosquito samples were processed for virus isolation in C6/36 cells. CHIKV isolate was characterised by RT-PCR - sequencing. Diagnostic levels of IgM antibodies were detected in 107 (49.3%) CHIKV samples - 22 (10.1%) DENV samples. IgM antibodies against JEV were not detected (n=46). Characterisation of the CHIKV isolate at genetic level demonstrated it as ECSA (E1: 226A). Thirty-six selected samples were also negative for Ross River, Sindbis, Murrey Valley - Hantan viruses. High prevalence of CHIKV IgM antibody positivity, clinical symptoms, virus isolation - the presence of vector mosquitoes clearly suggest CHIKV as the aetiological agent responsible for the outbreak.

Imprenta: Tropical Medicine & International Health, v. 16, n. 5, p. 585-588, 2011

Identificador do objeto digital: 10.1111/j.1365-3156.2011.02743.x

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2011

INDICADORES

ACESSO