Intracellular components associated with Chikungunya virus-specific ribonucleic acids in infected BHK21 cells
Autor(es): Igarashi A, Sasao F, Fukai K
Resumo: Cytoplasmic extracts of BHK21 cells infected with Chikungunya virus were analyzed by sucrose gradient sedimentation. Besides 140 S nucleocapsid, 65 S component associated with 26 S single-stranded ribonucleic acid was labeled with 3H-uridine in actinomycin-treated infected cells. The 65 S component, demonstrable in ethylenediaminetetraacetic acid-containing buffers, appeared to accumulate when protein synthesis was inhibited. After glutaraldehyde fixation, this component had a density of 1.48-1.50 g/ml in CsCl. Pulse-chase experiments did not indicate that the 65 S component was a precursor of the 140 S nucleocapsid.
Imprenta: Japanese Journal of Microbiology, v. 17, n. 5, p. 401-408, 1973
Identificador do objeto digital: 10.1111/j.1348-0421.1973.tb00791.x
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya Virus - Virus
Data de publicação: 1973
