Insights on profiling of phorbol, deoxyphorbol, ingenol and jatrophane diterpene esters by high performance liquid chromatography coupled to multiple stage mass spectrometry
Autor(es): Nothias-Scaglia Louis-Félix, Schmitz-Afonso Isabelle, Renucci Franck, Roussi Fanny, Touboul David, Costa Jean, Litaudon Marc, Paolini Julien
Resumo: This paper reports our effort to develop a comprehensive HPLC-MS(n)-based dereplication strategy for phorbol ester (PE), deoxyphorbol ester (dPE) - ingenol ester (IE) profiling in plant extracts. This strategy is composed of two sequential analysis exploiting specific hybrid triple quadrupole/linear ion trap instrument modes. A first run was performed using a multiple reaction monitoring (MRM) mode targeting fragmentation of PE - dPE/IE coupled with the acquisition of MS(2) spectrum for the ions at m/z 311 - m/z 313, respectively. A second run was then completed based on precursor ion scan mode (PIS) - automatic MS(2) acquisition for each quasimolecular ion. The developed approach was used to investigate ten Euphorbia extracts showing bioactivity against chikungunya virus replication. Experiments allowed partial annotation of three dPE/IE but no PE was detected. Results suggested that other types of diterpene esters displayed PE- - dPE/IE-like fragmentations. The study of jatrophane ester (JE) st-ards by CID fragmentation using low - high resolution mass spectrometry confirmed this hypothesis, highlighting challenges - difficulties of diterpene esters profiling within plant extracts. Nonetheless, the present LC-MS(n) method can be easily adapted to profile other types of diterpene esters.
Palavras-Chave: Deoxyphorbol; Diterpene esters; HPLC-MS(n); Ingenol; Jatrophane; Phorbol
Imprenta: Journal of Chromatography, v. 1422, p. 128-139, 2015
Identificador do objeto digital: 10.1016/j.chroma.2015.09.092
Descritores: Chikungunya Virus - Virus
Data de publicação: 2015
