Autor(es)Ekchariyawat Peeraya, Hamel Rodolphe, Bernard Eric, Wichit Sineewanlaya, Surasombatpattana Pornapat, Talignani Loïc, Thomas Frédéric, Choumet Valérie, Yssel Hans, Desprès Philippe, Briant Laurence, Missé Dorothée

ResumoArboviruses represent an emerging threat to human. They are transmitted to vertebrates by the bite of infected arthropods. Early transmission to vertebrates is initiated by skin puncture - deposition of virus in this organ. However, events at the bite site remain largely unknown. Here, we report that Chikungunya virus (CHIKV) - West Nile virus (WNV), despite belonging to distinct viral families, elicit a common antiviral signature in primary human dermal fibroblasts, attesting for the up regulation of interferon signaling pathways - leading to an increased expression of IFN-?, interleukins - chemokines. Remarkably, CHIKV - WNV enhance IL-1? expression - induce maturation of caspase-1, indicating the capacity of these pathogens to elicit activation of the inflammasome program in resident skin cells. CHIKV - WNV also induce the expression of the inflammasome sensor AIM2 in dermal fibroblasts, whereas inhibition of caspase-1 - AIM2 with siRNA interferes with both CHIKV- - WNV-induced IL-1? production by these cells. Finally, inhibition of the inflammasome via caspase-1 silencing was found to enhance CHIKV replication in dermal fibroblasts. Together, these results indicate that the skin contributes to the pro-inflammatory - anti-viral microenvironment via the activation of the inflammasome in the early stages following infection with arboviruses.

Palavras-ChaveAIM2; Arbovirus; Caspase 1; Chikungunya virus; Inflammasome; West Nile virus

ImprentaInfection, Genetics and Evolution, v. 32, p. 401-408, 2015

DescritoresChikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Immunology

Data de Publicação:2015