Autor(es): Gardner Joy, Rudd Penny A, Prow Natalie A, Belarbi Essia, Roques Pierre, Larcher Thibaut, Gresh Lionel, Balmaseda Angel, Harris Eva, Schroder Wayne A, Suhrbier Andreas

Resumo: Chikungunya virus (CHIKV) is a reemerging, ordinarily mosquito-transmitted, alphavirus that occasionally produces hemorrhagic manifestations, such as nose bleed - bleeding gums, in human patients. Interferon response factor 3 - 7 deficient (IRF3/7-/-) mice, which are deficient for interferon ?/? responses, reliably develop hemorrhagic manifestations after CHIKV infection. Here we show that infectious virus was present in the oral cavity of CHIKV infected IRF3/7-/- mice, likely due to hemorrhagic lesions in the olfactory epithelium that allow egress of infected blood into the nasal, - subsequently, oral cavities. In addition, IRF3/7-/- mice were more susceptible to infection with CHIKV via intranasal - oral routes, with IRF3/7-/- mice also able to transmit virus mouse-to-mouse without an arthropod vector. Cynomolgus macaques often show bleeding gums after CHIKV infection, - analysis of saliva from several infected monkeys also revealed the presence of viral RNA - infectious virus. Furthermore, saliva samples collected from several acute CHIKV patients with hemorrhagic manifestations were found to contain viral RNA - infectious virus. Oral fluids can therefore be infectious during acute CHIKV infections, likely due to hemorrhagic manifestations in the oral/nasal cavities.

Imprenta: PloS One, v. 10, n. 10, p. e0139481, 2015

Identificador do objeto digital: 10.1371/journal.pone.0139481

Descritores: Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2015