Autor(es): Tchankouo-Nguetcheu Stephane, Bourguet Edouard, Lenormand Pascal, Rousselle Jean-Claude, Namane Abdelkader, Choumet Valerie

Resumo: Arthropod-borne viral infections cause several emerging - resurging infectious diseases. Among the diseases caused by arboviruses, chikungunya is responsible for a high level of severe human disease worldwide. The salivary gl-s of mosquitoes are the last barrier before pathogen transmission. We undertook a proteomic approach to characterize the key virus/vector interactions - host protein modifications that occur in the salivary gl-s that could be responsible for viral transmission by using quantitative two-dimensional electrophoresis. We defined the protein modulations in the salivary gl-s of Aedes aegypti that were triggered 3 - 5 days after an oral infection (3 - 5 DPI) with chikungunya virus (CHIKV). Gel profile comparisons showed that CHIKV at 3 DPI modulated the level of 13 proteins, - at 5 DPI 20 proteins. The amount of 10 putatively secreted proteins was regulated at both time points. These proteins were implicated in blood-feeding or in immunity, but many have no known function. CHIKV also modulated the quantity of proteins involved in several metabolic pathways - in cell signalling. Our study constitutes the first analysis of the protein response of Aedes aegypti salivary gl-s infected with CHIKV. We found that the differentially regulated proteins in response to viral infection include structural proteins - enzymes for several metabolic pathways. Some may favour virus survival, replication - transmission, suggesting a subversion of the insect cell metabolism by arboviruses. For example, proteins involved in blood-feeding such as the short D7, an adenosine deaminase - inosine-uridine preferring nucleoside hydrolase, may favour virus transmission by exerting an increased anti-inflammatory effect. This would allow the vector to bite without the bite being detected. Other proteins, like the anti-freeze protein, may support vector protection.

Palavras-Chave: Aedes aegypti; Chikungunya virus; Mosquito salivary gland; Proteomics; Two-dimensional gel electrophoresis; Mass spectrometry

Imprenta: Parasites & Vectors, v. 5 , p. 264, 2012

Identificador do objeto digital: 10.1186/1756-3305-5-264

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Public health

Data de publicação: 2012

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