Autor(es): Sharma Anuj, Gupta Paridhi, Maheshwari Radha K

Resumo: Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia. In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect - virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis. INA completely inactivated CHIKV - can further be explored for developing an inactivated-CHIKV vaccine.

Palavras-Chave: Chikungunya virus; Inactivated vaccine; Iodonapthyl azide

Imprenta: Virology Journal, v. 9, p. 301, 2012

Identificador do objeto digital: 10.1186/1743-422X-9-301

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine

Data de publicação: 2012

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