Autor(es): Ziegler Sarah A, Nuckols John, McGee Charles E, Huang Yan-Jang Scott, Vanlandingham Dana L, Tesh Robert B, Higgs Stephen

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that in humans causes an acute febrile illness characterized by fever, arthralgia, - rash. It is currently associated with large outbreaks in Asia, Africa, - isl-s of the Indian Ocean - has been introduced from these tropical regions into Europe, where local transmission has been recorded on two occasions. The underlying basis of the pathogenesis of CHIKV - related alphaviruses that produce similar symptoms remains unclear. By applying new techniques, for example, in vivo imaging in live animals - arthropods, we may improve our underst-ing of viral pathogenesis in vertebrates - viral replication in mosquitoes. This technical report describes the evaluation of a CHIKV?luciferase clone to visualize infection - dissemination in both Aedes aegypti - Aedes albopictus mosquitoes - mice. In mosquitoes, luciferase activity was seen at 3 - 7 days post-infection in both head - abdomens. In vivo imaging of CHIKV-luciferase was detected in mice for up to 5 days post-infection at the site of inoculation with limited dissemination to the skeletal muscle.

Imprenta: Vector Borne and Zoonotic Diseases, v. 11, n. 11, p. 1471-1477, 2011

Identificador do objeto digital: 10.1089/vbz.2011.0648

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Public health

Data de publicação: 2011

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