Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti
Autor(es): van den Hurk Andrew F, Hall-Mendelin Sonja, Pyke Alyssa T, Frentiu Francesca D, McElroy Kate, Day Andrew, Higgs Stephen, O'Neill Scott L
Resumo: Incidence of disease due to dengue (DENV), chikungunya (CHIKV) - yellow fever (YFV) viruses is increasing in many parts of the world. The viruses are primarily transmitted by Aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. When transinfected into Ae. aegypti, the intracellular bacterium Wolbachia has recently been shown to inhibit replication of DENVs, CHIKV, malaria parasites - filarial nematodes, providing a potentially powerful biocontrol strategy for human pathogens. Because the extent of pathogen reduction can be influenced by the strain of bacterium, we examined whether the wMel strain of Wolbachia influenced CHIKV - YFV infection in Ae. aegypti. Following exposure to viremic blood meals, CHIKV infection - dissemination rates were significantly reduced in mosquitoes with the wMel strain of Wolbachia compared to Wolbachia-uninfected controls. However, similar rates of infection - dissemination were observed in wMel infected - non-infected Ae. aegypti when intrathoracic inoculation was used to deliver virus. YFV infection, dissemination - replication were similar in wMel-infected - control mosquitoes following intrathoracic inoculations. In contrast, mosquitoes with the wMelPop strain of Wolbachia showed at least a 10(4) times reduction in YFV RNA copies compared to controls. The extent of reduction in virus infection depended on Wolbachia strain, titer - strain of the virus, - mode of exposure. Although originally proposed for dengue biocontrol, our results indicate a Wolbachia-based strategy also holds considerable promise for YFV - CHIKV suppression.
Imprenta: PLoS Neglected Tropical Diseases, v. 6, n. 11, p. e1892, 2012
Identificador do objeto digital: 10.1371/journal.pntd.0001892
Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2012
