Immuno-chromatographic wicking assay for the rapid detection of Chikungunya viral antigens in mosquitoes (Diptera: Culicidae)
Autor(es): Hinson Juanita M, Davé Sonia, McMenamy Scott S, Davé Kirti, Turell Michael J
Resumo: The outbreak of disease caused by chikungunya virus (CHIKV) in 2006 - the recent spread of this virus to the Americas in 2013 indicate the potential for this virus to spread - cause significant disease. However, there are currently no accurate - reliable field-usable, diagnostic methods to provide critical, real-time information for early detection of CHIKV within the vector populations in order to implement appropriate vector control - personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect CHIKV in a pool of female Aedes mosquitoes containing a single CHIKV-infected mosquito. The CHIKV dipstick assay was simple to use, did not require a cold chain, - provided clear results within 1 h. It was highly specific - did not cross-react with samples spiked with a variety of other alpha, bunya, - flaviviruses. The CHIKV assay can provide real-time critical information on the presence of CHIKV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment - the focusing of vector control measures in high-risk areas.
Palavras-Chave: Arbovirus; Dipstick; Rapid detection; Surveillance; Wicking assay
Imprenta: Journal of Medical Entomology, v. 52, n. 4, p. 699-704, 2015
Identificador do objeto digital: 10.1093/jme/tjv047
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2015
