Identification of Asian genotype of Chikungunya virus isolated in Mexico
Autor(es): Díaz-Quiñonez José Alberto, Escobar-Escamilla Noé, Ortíz-Alcántara Joanna, Vázquez-Pichardo Mauricio, de la Luz Torres-Rodríguez María, Nuñez-León Alma, Torres-Longoria Belem, López-Martínez Irma, Ruiz-Matus Cuitláhuac, Kuri-Morales Pablo, Ramírez-González José Ernesto
Resumo: We identified 25 autochthonous chikungunya virus cases in Mexico, initially detected by RT-PCR targeting the E1 gene - propagated in C6/36 Aedes albopictus cells, in 2014. To determine the type of virus found, in a previous report, the genomes of 2 CHIKV strains were fully sequenced. Genome sequence analysis revealed that these isolates from Mexico belonged to the Asian genotype, - a phylogenetic association with the circulating strain in the British Virgin Isl-s was also established in the same year. This was further supported by changes in specific amino acids, E2-V368A - 6K-L20M. For these reasons, it can be inferred that the route of virus entry to Mexico was held across the countries in the Caribbean - Central America. The presence of E1-A226V mutation associated with more efficient replication in the salivary gl- of the A. albopictus mosquito was not observed. Interestingly, a newly acquired NSP4-S399C mutation was observed; however, the significance of changes in amino acid found in non-structural proteins in autochthonous strains remains to be elucidated.
Palavras-Chave: Chikungunya virus; Methagenomics; Mexico; Phylogeny
Imprenta: Virus Genes, v. 52, n. 1, p. 127-129, 2016
Identificador do objeto digital: 10.1007/s11262-015-1275-9
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Genome ; Chikungunya virus - Proteins ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus
Data de publicação: 2016
