Identical strength of the T cell responses against E2, nsP1 and capsid CHIKV proteins in recovered and chronic patients after the epidemics of 2005-2006 in La Reunion Island
Autor(es)Hoarau Jean-Jacques, Gay Frederick, Pellé Olivier, Samri Assia, Jaffar-B-jee Marie-Christine, Gasque Philippe, Autran Brigitte
ResumoTo characterize the immunity developed by patients infected by chikungunya virus (CHIKV), we studied the intensity - specificity of CHIKV-specific T cells mediated responses in chronic - recovered patients at 12 to 24 months post-infection. T cells were challenged in vitro against CHIKV synthetic peptides covering the length of three viral proteins, capsid, E2 - nsP1 proteins as well as all inactivated virus particles. Cytokine production was assessed by ELISPOT - intracellular labeling. T cells producing IFN-? were detected against CHIKV in 85% patient's cells either by direct ELISPOT assay (69% of patients) or after expansion of memory T cells allowing the detection of both CD4 - CD8 specific-T cells in 16% additional cases. The IFN-? response was mainly engaged in response to nsP1 or E2 (52% - 46% cases, respectively) but in only 27% cases against the capsid. The anti-E2 response represented half the magnitude of the total CHIKV IFN-? production - was mainly directed against the C-terminal half part of the protein. Almost all patients had conserved a T cell specific response against CHIKV with a clear hierarchy of T cell responses (CD8 > CD4) engaged against E2 > nsP1 > capsid. More importantly, the intensity of responses was not significantly different between recovered - chronic patients. These findings constitute key elements to a better underst-ing of patient T cell immunoreactivity against CHIKV - argue against a possible defect of T cell immunoresponse in the chronicity post-CHIKV infection.
ImprentaPloS One, v. 8, n. 12, p. e84695, 2013
Identificador do Objeto Digital10.1371/journal.pone.0084695
DescritoresChikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immunopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - T lymphocytes ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de Publicação:2013
