Human muscle satellite cells as targets of Chikungunya virus infection
Autor(es): Ozden Simona, Huerre Michel, Riviere Jean-Pierre, Coffey Lark L, Afonso Philippe V, Mouly Vincent, de Monredon Jean, Roger Jean-Christophe, El Amrani Mohamed, Yvin Jean-Luc, Jaffar Marie-Christine, Frenkiel Marie-Pascale, Sourisseau Marion, Schwartz Olivier, Butler-Browne Gillian, Desprès Philippe, Gessain Antoine, Ceccaldi Pierre-Emmanuel
Resumo: Chikungunya (CHIK) virus is a mosquito-transmitted alphavirus that causes in humans an acute infection characterised by fever, polyarthralgia, head-ache, - myalgia. Since 2005, the emergence of CHIK virus was associated with an unprecedented magnitude outbreak of CHIK disease in the Indian Ocean. Clinically, this outbreak was characterized by invalidating poly-arthralgia, with myalgia being reported in 97.7% of cases. Since the cellular targets of CHIK virus in humans are unknown, we studied the pathogenic events - targets of CHIK infection in skeletal muscle. Immunohistology on muscle biopsies from two CHIK virus-infected patients with myositic syndrome showed that viral antigens were found exclusively inside skeletal muscle progenitor cells (designed as satelllite cells), - not in muscle fibers. To evaluate the ability of CHIK virus to replicate in human satellite cells, we assessed virus infection on primary human muscle cells; viral growth was observed in CHIK virus-infected satellite cells with a cytopathic effect, whereas myotubes were essentially refractory to infection. This report provides new insights into CHIK virus pathogenesis, since it is the first to identify a cellular target of CHIK virus in humans - to report a selective infection of muscle satellite cells by a viral agent in humans.
Imprenta: PloS One, v. 2, n. 6, p. e527, 2007
Identificador do objeto digital: 10.1371/journal.pone.0000527
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2007
