Host alternation of Chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures
Autor(es): Coffey Lark L, Vignuzzi Marco
Resumo: The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve - maintain the ability to infect vertebrate - invertebrate hosts are poorly understood. To underst- how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate - vertebrate cells (invertebrate ? vertebrate) to simulate natural alternation - contrasted the results with those for populations that were artificially released from cycling by passage in single cell types. These CHIKV populations were characterized by measuring genetic diversity, changes in fitness, - adaptability to novel selective pressures. The greatest fitness increases were observed in alternately passaged CHIKV, without drastic changes in population diversity. The greatest increases in genetic diversity were observed after serial passage - correlated with greater adaptability. These results suggest an evolutionary trade-off between maintaining fitness for invertebrate ? vertebrate cell cycling, where maximum adaptability is possible only via enhanced population diversity - extensive exploration of sequence space.
Imprenta: Journal of Virology, v. 85, n. 2, p. 1025-1035, 2011
Identificador do objeto digital: 10.1128/JVI.01918-10
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya Virus - Virus
Data de publicação: 2011
