HLA class II allele polymorphism in an outbreak of Chikungunya fever in Middle Andaman, India
Autor(es): Chaaithanya Itta Krishna, Muruganandam Nagarajan, Anwesh Maile, Rajesh Reesu, Ghosal Sruti R, Kartick Chinnaiah, Prasad Kadiyala Nageswara, Muthumani Karuppiah, Vijayachari Paluru
Resumo: A sudden upsurge of fever cases with joint pain was observed in the outpatient department, Community Health Centre, Rangat during July-August 2010 in Rangat Middle -aman, India. The aetiological agent responsible for the outbreak was identified as chikungunya virus (CHIKV), by using RT-PCR - IgM ELISA. The study investigated the association of polymorphisms in the human leucocyte antigen class II genes with susceptibility or protection against CHIKV. One hundred - one patients with clinical features suggestive of CHIKV infection - 104 healthy subjects were included in the study. DNA was extracted - typed for HLA-DRB1 - DQB1 alleles. Based on the amino acid sequences of HLA-DQB1 retrieved from the IMGT/HLA database, critical amino acid differences in the specific peptide-binding pockets of HLA-DQB1 molecules were investigated. The frequencies of HLA-DRB1 alleles were not significantly different, whereas lower frequency of HLA-DQB1*03:03 was observed in CHIKV patients compared with the control population [P = 0·001, corrected P = 0·024; odds ratio (OR) = 0, 95% confidence interval (95% CI) 0·0-0·331; Peto's OR = 0·1317, 95% CI 0·0428-0·405). Significantly lower frequency of glutamic acid at position 86 of peptide-binding pocket 1 coding HLA-DQB1 genotypes was observed in CHIKV patients compared with healthy controls (P = 0·004, OR = 0·307, 95% CI 0·125-0·707). Computational binding predictions of CD4 epitopes of CHIKV by NetMHCII revealed that HLA-DQ molecules are known to bind more CHIKV peptides than HLA-DRB1 molecules. The results suggest that HLA-DQB1 alleles - critical amino acid differences in the peptide-binding pockets of HLA-DQB1 alleles might have role in influencing infection - pathogenesis of CHIKV.
Palavras-Chave: Andaman Islands; HLA; Chikungunya; Chikungunya virus; Immunogenetics
Imprenta: Immunology, v. 140, n. 2, p. 202-210, 2013
Identificador do objeto digital: 10.1111/imm.12128
Descritores: Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2013
