Global transcriptional dynamics of diapause induction in non-blood-fed and blood-fed Aedes albopictus
Autor(es): Huang Xin, Poelchau Monica F, Armbruster Peter A
Resumo: Aedes albopictus is a vector of increasing public health concern due to its rapid global range expansion - ability to transmit Dengue virus, Chikungunya virus - a wide range of additional arboviruses. Traditional vector control strategies have been largely ineffective against Ae. albopictus - novel approaches are urgently needed. Photoperiodic diapause is a crucial ecological adaptation in a wide range of temperate insects. Therefore, targeting the molecular regulation of photoperiodic diapause or diapause-associated physiological processes could provide the basis of novel approaches to vector control. We investigated the global transcriptional profiles of diapause induction in Ae. albopictus by performing paired-end RNA-Seq of biologically replicated libraries. We sequenced RNA from whole bodies of adult females reared under diapause-inducing - non-diapause-inducing photoperiods either with or without a blood meal. We constructed a comprehensive transcriptome assembly that incorporated previous assemblies - represents over 14,000 annotated dipteran gene models. Mapping of sequence reads to the transcriptome identified differential expression of 2,251 genes in response to diapause-inducing short-day photoperiods. In non-blood-fed females, potential regulatory elements of diapause induction were transcriptionally up-regulated, including two of the canonical circadian clock genes, timeless - cryptochrome 1. In blood-fed females, genes in metabolic pathways related to energy production - offspring provisioning were differentially expressed under diapause-inducing conditions, including the oxidative phosphorylation pathway - lipid metabolism genes. This study is the first to utilize powerful RNA-Seq technologies to elucidate the transcriptional basis of diapause induction in any insect. We identified c-idate genes - pathways regulating diapause induction, including a conserved set of genes that are differentially expressed as part of the diapause program in a diverse group of insects. These genes provide c-idates whose diapause-associated function can be further interrogated using functional genomics approaches in Ae. albopictus - other insects.
Imprenta: PLoS Neglected Tropical Diseases, v. 9, n. 4, p. e0003724, 2015
Identificador do objeto digital: 10.1371/journal.pntd.0003724
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2015
