Germline excision of transgenes in Aedes aegypti by homing endonucleases
Autor(es): Aryan Azadeh, Anderson Michelle A E, Myles Kevin M, Adelman Zach N
Resumo: Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) - chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-str-ed DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI - I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-str- annealing (SSA) - non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing - gene drive strategies in Ae. aegypti, - confirm the utility of HEs in the manipulation - modification of transgenes in this important vector.
Imprenta: Scientific Reports, v. 3, p. 1603, 2013
Identificador do objeto digital: 10.1038/srep01603
Descritores: Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue
Data de publicação: 2013
