Genomic assays for identification of Chikungunya virus in blood donors, Puerto Rico, 2014
Autor(es): Chiu Charles Y, Bres Vanessa, Yu Guixia, Krysztof David, Naccache Samia N, Lee Deanna, Pfeil Jacob, Linnen Jeffrey M, Stramer Susan L
Resumo: A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, - next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.
Palavras-Chave: ViroChip microarray; Blood donor; Chikungunya virus; Next-generation sequencing; Transcription-mediated amplification; Transfusion-transmitted infection; Viruses; Whole-genome sequencing
Imprenta: Emerging Infectious Diseases, v. 21, n. 8, p. 1409-1413, 2015
Identificador do objeto digital: 10.3201/eid2108.150458
Descritores: Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology
Data de publicação: 2015
