Genetic characterization of E2 region of Chikungunya virus circulating in Odisha, Eastern India from 2010 to 2011
Autor(es): Sahu Abhipsa, Das Biswadeep, Das Mumani, Patra Aparna, Biswal Sudarsan, Kar Santanu K, Hazra Rupenangshu K
Resumo: Chikungunya virus (CHIKV) infection has caught attention yet again as it rages around the globe affecting millions of people. The virus caused epidemic outbreaks affecting more than 15,000 people in Odisha, Eastern India since 2010. In this study, complete genetic characterization of E2 gene of CHIKV circulating in Odisha from 2010 to 2011 was performed by virus isolation, RT-PCR, molecular phylogenetics - bioinformatics methods. Phylogenetic analyses revealed the circulation of Indian Ocean Lineage (IOL) strains of ECSA genotype of CHIKV in Odisha. Several mutations were detected in the E2 gene, viz. E2-R82G, E2-L210Q, E2-I211T, E2-V229I - E2-S375T which had various adaptive roles during the evolution of CHIKV. The CHIKV E2 peptide ??KTDDSHD?³ was predicted to be the most probable T-cell epitope - peptide ??FVRTSAPCT?² predicted to be the common T - B cell epitope having high antigenicity. The amino acid positions 356-379 - 365-385 were predicted to be transmembrane helical domains - indicated E2 protein anchorage in intracellular membranes for effective interaction with the host receptors. Positive selection pressure was observed in five specific sites, 210, 211, 318, 375, - 377 which were observed to be fixed advantageously in most viral isolates. Structural modeling revealed that E2 gene of CHIKV was composed of 3 domains - the major adaptive mutations were detected in domain B, which can modulate binding of CHIKV to host cells, while the transmembrane domain in domain C - the epitopes were located in domain A, which was found to be most conserved. This is the first report from Eastern India demonstrating a predictive approach to the genetic variations, epitopic regions - the transmembrane helices of the E2 region. The results of this study, combined with other published observations, will exp- our knowledge about the E2 region of CHIKV which can be exploited to develop control measures against CHIKV.
Palavras-Chave: Chikungunya; Genetic analysis; Positive selection; Structural modeling; T cell epitope; Transmembrane helix
Imprenta: Infection, Genetics and Evolution, v. 18, p. 113-124, 2013
Identificador do objeto digital: 10.1016/j.meegid.2013.04.037
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Cell ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health
Data de publicação: 2013
