Autor(es): Fafetine J M, Domingos A, Antunes S, Esteves A, Paweska J T, Coetzer J A W, Rutten V P M G, Neves L

Resumo: Due to the unpredictable - explosive nature of Rift Valley fever (RVF) outbreaks, rapid - accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced - characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source - from inactivated RVFV, in Western blot analysis - in an enzyme-linked immunosorbent assay (ELISA). Cross-reactivity with genetically related - non-related arboviruses including Bunyamwera - Calovo viruses (Bunyaviridae family), West Nile - Dengue-2 viruses (Flaviviridae family), - Sindbis - Chikungunya viruses (Togaviridae family) was not detected. These MAbs represent a useful tool for the development of rapid diagnostic assays for early recognition of RVF.

Palavras-Chave: Rift Valley fever virus; Cross-reactivity; Monoclonal antibodies; Nucleocapsid protein

Imprenta: Transboundary and Emerging Diseases, v. 60, supl 2, p. 24-30, 2013

Identificador do Objeto Digital: 10.1111/tbed.12140

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology

Data de Publicação: 2013