Autor(es): Moyen Nanikaly, Thiberville Simon-Djamel, Pastorino Boris, Nougairede Antoine, Thirion Laurence, Mombouli Jean-Vivien, Dimi Yannick, Leparc-Goffart Isabelle, Capobianchi Maria Rosaria, Lepfoundzou Amelia Dzia, de Lamballerie Xavier

Resumo: Chikungunya is an Aedes -borne disease characterised by febrile arthralgia - responsible for massive outbreaks. We present a prospective clinical cohort study - a retrospective serological study relating to a CHIK outbreak, in the Republic of Congo in 2011. We analysed 317 suspected cases, of which 308 (97.2%) lived in the city of Brazzaville (66.6% in the South area). Amongst them, 37 (11.7%) were CHIKV+ve patients (i.e., biologically confirmed by a real-time RT-PCR assay), of whom 36 (97.3%) had fever, 22 (66.7%) myalgia - 32 (86.5%) arthralgia. All tested negative for dengue. The distribution of incident cases within Brazzaville districts was compared with CHIKV seroprevalence before the outbreak (34.4% in 517 blood donors), providing evidence for previous circulation of CHIKV. We applied a CHIK clinical score to 126 patients recruited within the two first day of illness (including 28 CHIKV+ves (22.2%)) with sensitivity (78.6%) - specificity (72.4%) values comparing with those of the referent study in Reunion Isl-. The negative predictive value was high (92%), but the positive predictive value (45%) indicate poor potential contribution to medical practice to identify CHIKV+ve patients in low prevalence outbreaks. However, the score allowed a slightly more accurate follow-up of the evolution of the outbreak than the criterion fever+arthralgia". The complete sequencing of a Congolase isolate (Brazza_MRS1) demonstrated belonging to the East/Central/South African lineage - was further used for producing a robust genome-scale CHIKV phylogenetic analysis. We describe the first Chikungunya outbreak declared in the Republic of Congo. The seroprevalence study conducted amongst blood donors before outbreak provided evidence for previous CHIKV circulation. We suggest that a more systematic survey of the entomological situation - of arbovirus circulation is necessary in Central Africa for better underst-ing the environmental, microbiological - sociological determinants of emergence."

Imprenta: PloS One, v. 9, n. 12, p. e115938, 2014

Identificador do objeto digital: 10.1371/journal.pone.0115938

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2014

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