Autor(es): Vazeille Marie, Mousson Laurence, Failloux Anna-Bella

Resumo: Aedes albopictus was responsible for transmission in the first outbreak of chikungunya (CHIK) on La Réunion Isl-, Indian Ocean, in 2005-2006. The magnitude of the outbreak on this isl-, which had been free of arboviral diseases for over 30 years, as well as the efficiency of Ae. albopictus as the main vector, raises questions about the maintenance of the CHIK virus (CHIKV) through vertical transmission mechanisms. Few specimens collected from the field as larvae were found to be infected. In this study, Ae. albopictus originating from La Réunion were orally infected with a blood-meal containing 10(8) pfu/mL of the CHIKV epidemic strain (CHIKV 06.21). Eggs from the first - second gonotrophic cycles were collected - raised to the adult stage. The infectious status of the progeny was checked (i) by immunofluorescence on head squashes of individual mosquitoes to detect the presence of viral particles or (ii) by quantitative RT-PCR on mosquito pools to detect viral RNA. We analysed a total of 1,675 specimens from the first gonotrophic cycle - 1,709 from the second gonotrophic cycle without detecting any viral particles or viral RNA. These laboratory results are compared to field records.

Palavras-Chave: Vertical transmission; Chikungunya virus; Aedes albopictus; La Réunion Island; Indian Ocean

Imprenta: Memo?rias do Instituto Oswaldo Cruz, v. 104, n. 4, p. 632-635, 2009

Identificador do objeto digital: 10.1590/S0074-02762009000400017

Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health

Data de publicação: 2009

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