External quality assessment studies for laboratory performance of molecular and serological diagnosis of Chikungunya virus infection
Autor(es): Jacobsen Sonja, Patel Pranav, Schmidt-Chanasit Jonas, Leparc-Goffart Isabelle, Teichmann Anette, Zeller Herve, Niedrig Matthias
Resumo: Since the re-emergence of Chikungunya virus (CHIKV) in Reunion in 2005 - the recent outbreak in the Caribbean isl-s with an expansion to the Americas the CHIK diagnostic became very important. We evaluate the performance of laboratories regarding molecular - serological diagnostic of CHIK worldwide. A panel of 12 samples for molecular - 13 samples for serology were provided to 60 laboratories in 40 countries for evaluating the sensitivity - specificity of molecular - serology testing. The panel for molecular diagnostic testing was analysed by 56 laboratories returning 60 data sets of results whereas the 56 - 60 data sets were returned for IgG - IgM diagnostic from the participating laboratories. Twenty-three from 60 data sets performed optimal, 7 acceptable - 30 sets of results require improvement. From 50 data sets only one laboratory shows an optimal performance for IgM detection, followed by 9 data sets with acceptable - the rest need for improvement. From 46 IgG serology data sets 20 provide an optimal, 2 an acceptable - 24 require improvement performance. The evaluation of some of the diagnostic performances allows linking the quality of results to the in-house methods or commercial assays used. The external quality assurance for CHIK diagnostics provides a good overview on the laboratory performance regarding sensitivity - specificity for the molecular - serology diagnostic required for the quick - reliable analysis of suspected CHIK patients. Nearly half of the laboratories have to improve their diagnostic profile to achieve a better performance.
Palavras-Chave: CHIK; Chikungunya; Diagnosis; EQA; Molecular; Proficiency test; Quality control; Serology
Imprenta: Journal of Clinical Virology, v. 76, p. 55-65, 2016
Identificador do objeto digital: 10.1016/j.jcv.2016.01.008
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Serology ; Chikungunya virus - Molecular methods ; Chikungunya virus - Serological diagnosis ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever
Data de publicação: 2016
