Autor(es): Patil Dilip R, Hundekar Supriya L, Arankalle Vidya A

Resumo: During the recent re-emergence of chikungunya, clinical complications - deaths were recorded. Persistent musculoskeletal pain, arthralgia, arthritis were among the most common complications. To underst- pathogenesis of CHIKV induced disease, we developed suckling, outbred mouse model presenting with severe myopathology. Histopathology, dynamics of viral load, IgG antibodies/isotypes, serum cytokines by cytometric bead array - mRNA expression levels of immune response genes in the target tissue by Taqman Low Density Array were studied. Peak viral load was associated with peak serum levels of CCL-2,KC, CCL-4, RANTES, IL-6, IL-10, CSF-3, - locally very high mRNA expression of CCL-2, CXCL-10, CXCL-11 - concomitant IFN?, IL-10, STAT-1, SOCS-1 - CSF-3 suggesting strong IFN? program. Symptomatic phase correlated with peak serum levels of IL-2, IFN?, IL-17, CCL-3, IL-1?, eotaxin, IL-9 - CSF-2 - locally with peak mRNA expression of macrophage induced pro inflammatory cytokines - immune infiltration biased towards Th1. IgG antibodies were detected on day 6PI, reaching high titres by day 11PI. IgG2a was the predominant isotype, indicating Th1 bias. This is the first report of comprehensive analysis of immune response genes expression in target tissue of CHIKV mouse model. The data would contribute significantly in underst-ing pathogenesis of CHIKV disease - viral myopathies.

Palavras-Chave: Chikungunya; Immune response genes; Serum cytokines; Mouse model; Muscle pathology

Imprenta: Microbes and Infection, v. 14, n. 5, p. 457-469, 2012

Identificador do objeto digital: 10.1016/j.micinf.2011.12.008

Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Cytopathology ; Chikungunya virus - Genome ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology

Data de publicação: 2012