Autor(es)Cho Byungki, Kim Jungho, Cho Jang-Eun, Jeon Bo-Young, Park Sun

ResumoChikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIK fever, which typically manifests as a febrile illness. To develop a CHIKV-specific diagnostic test, CHIKV capsid protein was expressed using a baculovirus expression system. The seroreactvity of the recombinant CHIKV capsid protein was evaluated by ELISA - immuochromatographic assay (ICA), using 40 anti-CHIKV-positive - 20 anti-CHIKV-negative sera, an additional 20 normal sera samples from healthy Koreans, - 20 anti-Dengue virus sera samples. The sensitivity of the recombinant CHIKV capsid protein was 85% - 87.5% as measured by ELISA - ICA, respectively. The specificity of the recombinant CHIKV capsid protein was 100% both by ELISA - by ICA. No cross-reactivity of the capsid protein was seen with anti-Dengue virus sera samples. There was a significant correlation between the ELISA- - ICA-measured seroreactivities of the recombinant CHIKV capsid protein for anti-CHIKV IgM-positive sera samples. These results suggest that the recombinant CHIKV capsid protein could be used in a diagnostic test for identifying CHIKV disease.

Palavras-ChaveChikungunya virus (CHIKV); Capsid protein; Baculovirus; ELISA; Immunochromatographic assay (ICA)

ImprentaJournal of Virological Methods, v. 154, n. 1-2, p. 154-159, 2008

DescritoresChikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de Publicação:2008