Autor(es): Cho Byungki, Jeon Bo-Young, Kim Jungho, Noh Jaesang, Kim Jiha, Park Minjung, Park Sun

Resumo: Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe- transmitted viral disease in Africa, India, South-East Asia, - recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), - indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. CHIKV E1, - E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 - E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA. The recombinant CHIKV E1 - E2 envelope protein showed sensitivity of 77.5% - 90%, respectively. The specificities of both CHIKV E1 - E2 envelope proteins were 100%. The recombinant CHIKV E1 - E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.

Palavras-Chave: Chikungunya virus; E1 and E2; Envelope protein; Baculovirus; Indirect IgM ELISA

Imprenta: Yonsei Medical Journal, v. 49, n. 5, p. 828-835, 2008

Identificador do objeto digital: 10.3349/ymj.2008.49.5.828 

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - Serological diagnosis ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2008

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