Exploiting mosquito sugar feeding to detect mosquito-borne pathogens
Autor(es): Hall-Mendelin Sonja, Ritchie Scott A, Johansen Cheryl A, Zborowski Paul, Cortis Giles, Dandridge Scott, Hall Roy A, van den Hurk Andrew F
Resumo: Arthropod-borne viruses (arboviruses) represent a global public health problem, with dengue viruses causing millions of infections annually, while emerging arboviruses, such as West Nile, Japanese encephalitis, - chikungunya viruses have dramatically exp-ed their geographical ranges. Surveillance of arboviruses provides vital data regarding their prevalence - distribution that may be utilized for biosecurity measures - the implementation of disease control strategies. However, current surveillance methods that involve detection of virus in mosquito populations or sero-conversion in vertebrate hosts are laborious, expensive, - logistically problematic. We report a unique arbovirus surveillance system to detect arboviruses that exploits the process whereby mosquitoes expectorate virus in their saliva during sugar feeding. In this system, infected mosquitoes captured by CO(2)-baited updraft box traps are allowed to feed on honey-soaked nucleic acid preservation cards within the trap. The cards are then analyzed for expectorated virus using real-time reverse transcription-PCR. In field trials, this system detected the presence of Ross River - Barmah Forest viruses in multiple traps deployed at two locations in Australia. Viral RNA was preserved for at least seven days on the cards, allowing for long-term placement of traps - continuous collection of data documenting virus presence in mosquito populations. Furthermore no mosquito h-ling or processing was required - cards were conveniently shipped to the laboratory overnight. The simplicity - efficacy of this approach has the potential to transform current approaches to vector-borne disease surveillance by streamlining the monitoring of pathogens in vector populations.
Imprenta: Proceedings of the National Academy of Sciences of the United States of America, v. 107, n. 25, p. 11255-11259, 2010
Identificador do objeto digital: 10.1073/pnas.1002040107
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2010
