Evidence for homologous recombination in Chikungunya virus
Autor(es): Casal Pablo E, Chouhy Diego, Bolatti Elisa M, Perez Germán R, Stella Emma J, Giri Adriana A
Resumo: Chikungunya Virus (CHIKV), a mosquito-transmitted alphavirus, causes acute fever - joint pain in humans. Recently, endemic CHIKV infection outbreaks have jeopardized public health in wider geographical regions. Here, we analyze the phylogenetic associations of CHIKV - explore the potential recombination events on 152 genomic isolates deposited in GenBank database. The CHIKV genotypes [West African, Asian, East/Central/South African (ECSA)], - a clear division of ECSA clade into three sub-groups (I-II-III), were defined by Bayesian analysis; similar results were obtained using E1 gene sequences. A nucleotide identity-based approach is provided to facilitate CHIKV classification within ECSA clade. Using seven methods to detect recombination, we found a statistically significant event (p-values range: 1.14×10(-7)-4.45×10(-24)) located within the nsP3 coding region. This finding was further confirmed by phylogenetic networks (PHI Test, p=0.004) - phylogenetic tree incongruence analysis. The recombinant strain, KJ679578/India/2011 (ECSA III), derives from viruses of ECSA III - ECSA I. Our study demonstrates that recombination is an additional mechanism of genetic diversity in CHIKV that might assist in the cross-species transmission process.
Palavras-Chave: Bayesian phylogeny; Chikungunya virus; Complete ORFs/E1 gene; East/Central/South African clade; Nucleotide identity classification; Recombination
Imprenta: Molecular Phylogenetics and Evolution, v. 85, p. 68-75, 2015
Identificador do objeto digital: 10.1016/j.ympev.2015.01.016
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Public health
Data de publicação: 2015
