Evaluation of multiple antigenic peptides based on the Chikungunya E2 protein for improved serological diagnosis of infection
Autor(es): Bhatnagar Santwana, Kumar Pradeep, Mohan Teena, Verma Priyanka, Parida M M, Hoti S L, Rao D N
Resumo: In recent years, Chikungunya virus (CHIKV) reemerged - numerous outbreaks were reported all over the world. After screening CHIKV-positive sera, we had already reported many dominant epitopes within the envelope E2 protein of CHIKV. In the present study, we aimed at developing a highly sensitive immunodiagnostic assay for CHIKV based on a multiple antigenic peptide (MAP) approach using selective epitopes of the E2 protein. MAPs in four different E2 peptide combinations were screened with CHIKV-positive sera. The MAPs reacted with all CHIKV-positive sera - no reactivity was seen with healthy or dengue-positive sera. Our results indicate that MAP 1 seems to be an alternate antigen to full-length protein E2 for immunodiagnosis of CHIKV infections with high sensitivity - specificity.
Imprenta: Viral Immunology, v. 28, n. 2, p. 107-112, 2015
Identificador do objeto digital: 10.1089/vim.2014.0031
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serological diagnosis ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
