Autor(es): Rianthavorn Pornpimol, Wuttirattanakowit Norra, Prianantathavorn Kesmanee, Limpaphayom Noppachart, Theamboonlers Apiradee, Poovorawan Yong

Resumo: Chikungunya (CHIK) is a re-emerging disease causing a large negative impact on global health - economics. Clinical manifestations of CHIK are non specific - difficult to differentiate from dengue hemorrhagic fever or other viral exanthema. A rapid, simple - reliable diagnostic assay is necessary for CHIK outbreak control especially in countries with insufficient access to well-equipped laboratories. The aim of the study is to evaluate a commercially rapid, qualitative CHIK diagnostic assay based on specific IgM antibody detection. Performance of the rapid assay was evaluated in comparison with semi-nested RT-PCR - IgM detection by ELISA. The sensitivity of the rapid assay was not constant but positively correlated with duration of symptoms. If the test was conducted within the first week, sensitivity - specificity was 22% - 88%, respectively. If the patients were tested after the first week, sensitivity was increased to 83% while specificity was decreased to 71%. Thus, the rapid assay should not be used as a screening tool during the first week of CHIK due to its low sensitivity.

Palavras-Chave: Sensitivity; Specificity; Chikungunya; Rapid test; ELISA; RT-PCR

Imprenta: The Southeast Asian Journal of Tropical Medicine and Public Health, v. 41, n. 1, p. 92-96, 2010

Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2010

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