Autor(es): Ho Phui San, Ng Mary Mah Lee, Chu Justin Jang Hann

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus - one of the prevalent re-emerging arbovirus in tropical - subtropical regions of Asia, Africa, - Central - South America. It produces a spectrum of illness ranging from inapparent infection to moderate febrile illness as well as severe arthralgia or arthritis affecting multiple joints. In this study, a quantitative, one-step real-time SYBR Green-based RT-PCR system for the non-structural protein 2 (nsP2) of CHIKV that can quantify a wide range of viral RNA concentrations was developed. Comparisons between the conventional semi-quantitative RT-PCR assay, immunofluorescence detection method - the one-step SYBR Green-based RT-PCR assay in the detection of CHIKV infection revealed much rapid - increase sensitivity of the latter method. Furthermore, this newly developed assay was validated by in vitro experiments in which ribavirin, a well-known RNA virus inhibitor, showed a dose-dependent inhibition of virus replication on cells that was assessed by viral infectivity - viral RNA production. Our results demonstrate the potential of this newly developed one-step SYBR Green I-based RT-PCR assay may be a useful tool in rapid detection of CHIKV - monitoring the extent of viral replication possibly in patients' samples.

Imprenta: Virology Journal, v. 7, p. 13, 2010

Identificador do objeto digital: 10.1186/1743-422X-7-13

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Public health

Data de publicação: 2010

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