Autor(es): Coffey Lark L, Page Brady L, Greninger Alexander L, Herring Belinda L, Russell Richard C, Doggett Stephen L, Haniotis John, Wang Chunlin, Deng Xutao, Delwart Eric L

Resumo: Viral metagenomics characterizes known - identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. Sequence comparisons revealed strains of Liao Ning virus (Reovirus, Seadornavirus), previously detected only in China, livestock-infecting Stretch Lagoon virus (Reovirus, Orbivirus), two novel dimarhabdoviruses, named Beaumont - North Creek viruses, - two novel orthobunyaviruses, named Murrumbidgee - Salt Ash viruses. The novel virus proteomes diverged by ? 50% relative to their closest previously genetically characterized viral relatives. Deep sequencing also generated genomes of Warrego - Wallal viruses, orbiviruses linked to kangaroo blindness, whose genomes had not been fully characterized. This study highlights viral metagenomics in concert with traditional arbovirus surveillance to characterize known - new arboviruses in field-collected mosquitoes. Follow-up epidemiological studies are required to determine whether the novel viruses infect humans.

Palavras-Chave: Virus discovery; Deep sequencing; Arbovirus surveillance; Bunyavirus; Rhabdovirus; Reovirus; Novel virus; Mosquito; Australia

Imprenta: Virology, v. 448, p. 146-158, 2014

Identificador do objeto digital: 10.1016/j.virol.2013.09.026

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2014

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