Autor(es): Stapleford Kenneth A, Coffey Lark L, Lay Sreyrath, Bordería Antonio V, Duong Veasna, Isakov Ofer, Rozen-Gagnon Kathryn, Arias-Goeta Camilo, Blanc Hervé, Beaucourt Stéphanie, Halilo?lu Türkan, Schmitt Christine, Bonne Isabelle, Ben-Tal Nir, Shomron Noam, Failloux Anna-Bella, Buchy Philippe, Vignuzzi Marco

Resumo: The high replication - mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict - prevent epidemics. By studying the spatial - temporal evolution of chikungunya virus during natural transmission between mosquitoes - mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut - salivary gl- pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya - identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito - mammalian hosts by altering virion stability - fusogenic activity. Thus, virus evolutionary trajectories can be predicted - studied in the short term before new variants displace currently circulating strains.

Imprenta: Cell Host & Microbe, v. 15, n. 6, p. 706-716, 2014

Identificador do objeto digital: 10.1016/j.chom.2014.05.008

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biochemistry ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2014

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