Autor(es): Nasar Farooq, Erasmus Jesse H, Haddow Andrew D, Tesh Robert B, Weaver Scott C

Resumo: Most alphaviruses are mosquito-borne - exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, - humans. Occasionally, alphaviruses can spill over into the human population - cause disease characterized by debilitating arthralgia or fatal encephalitis. Recently, a unique alphavirus, Eilat virus (EILV), was described that readily infects mosquito but not vertebrate cell lines. Here, we investigated the ability of EILV to induce superinfection exclusion. Prior infection of C7/10 (Aedes albopictus) cells with EILV induced homologous - heterologous interference, reducing the virus titers of heterologous superinfecting viruses (SINV, VEEV, EEEV, WEEV, - CHIKV) by ~10-10,000 fold - delaying replication kinetics by 12-48h. Similar to in vitro infection, prior in vivo EILV infection of Aedes aegypti mosquitoes delayed dissemination of chikungunya virus for 3 days. This is the first evidence of heterologous interference induced by a mosquito-specific alphavirus in vitro - in vivo.

Palavras-Chave: Alphavirus; Eilat virus; Superinfection

Imprenta: Virology, v. 484, p. 51-58, 2015

Identificador do objeto digital: 10.1016/j.virol.2015.05.009

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus

Data de publicação: 2015

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