Effects of infectious virus dose and bloodmeal delivery method on susceptibility of Aedes aegypti and Aedes albopictus to Chikungunya virus
Autor(es): Pesko Kendra, Westbrook Catherine J, Mores Christopher N, Lounibos L Philip, Reiskind Michael H
Resumo: Chikungunya virus (CHIKV) is an arbovirus (genus Alphavirus, family Togaviridae) that has recently caused disease outbreaks in the Indian Ocean basin - southern Europe. These outbreaks could be associated with a possible shift in primary vector from Aedes aegypti to Ae. albopictus. To evaluate vector competence differences in possible CHIKV vectors, we evaluated the dose-dependant susceptibility of Florida strains of Ae. albopictus - Ae. aegypti for infection with a La Réunion isl- strain of CHIKV. Pledget - water-jacketed membrane feeding systems were also evaluated. We show that both Aedes spp. were susceptible to the highest CHIKV doses, whereas only Ae. albopictus developed disseminated infections after exposure to the two lowest doses. Infection rates for both mosquito species were significantly affected by the bloodmeal delivery method used. This information is important in assessing risk of an outbreak of imported CHIKV in the United States, in determining differences in vectorial capacity of these two vector species, - in evaluating arbovirus delivery methods in the laboratory.
Palavras-Chave: Arbovirus; Viremia; Vector competence; Vectorial capacity; Blood feeding
Imprenta: Journal of Medical Entomology, v. 46, n. 2, p. 395-359, 2009
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission
Data de publicação: 2009
