Effective cutaneous vaccination using an inactivated Chikungunya virus vaccine delivered by Foroderm
Autor(es): Rudd Penny A, Raphael Anthony P, Yamada Miko, Nufer Kaitlin L, Gardner Joy, Le Thuy T T, Prow Natalie A, Dang Nhung, Schroder Wayne A, Prow Tarl W, Suhrbier Andreas
Resumo: Foroderm is a new cutaneous delivery technology that uses high-aspect ratio, cylindrical silica microparticles, that are massaged into the skin using a 3D-printed microtextured applicator, in order to deliver payloads across the epidermis. Herein we show that this technology is effective for delivery of a non-adjuvanted, inactivated, whole-virus chikungunya virus vaccine in mice, with minimal post-vaccination skin reactions. A single topical Foroderm-based vaccination induced T cell, Th1 cytokine - antibody responses, which provided complete protection against viraemia - disease after challenge with chikungunya virus. Foroderm vaccination was shown to deliver fluorescent, virus-sized beads across the epidermis, with beads subsequently detected in draining lymph nodes. Foroderm vaccination also stimulated the egress of MHC II(+) antigen presenting cells from the skin. Foroderm thus has potential as a simple, cheap, effective, generic, needle-free technology for topical delivery of vaccines.
Palavras-Chave: Chikungunya virus; Cutaneous vaccination; Foroderm; Vaccine delivery
Imprenta: Vaccine, v. 33, n. 39, p. 5172-5180, 2015
Identificador do objeto digital: 10.1016/j.vaccine.2015.07.099
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Inflammation ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine
Data de publicação: 2015
